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V5-Tag Monoclonal Antibody

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產(chǎn)品詳情相關(guān)產(chǎn)品

V5-Tag Monoclonal Antibody

貨號(hào)：

HYK-200161
規(guī)格：
圖片：
IP antibody use: 5μg V5 Mouse IgG1 per ml Lysate, WB 1: 5000
Lane 1: untransfected 293 cell lysate
Lane 2: transfected 293 cell lysate with V5-tag fusion protein
Lane 3: IP (untransfted 293 + anti-V5 mAb + Protein G agarose)
Lane 4: IP (transfected 293 + normal Mouse IgG + Protein G agarose)
Lane 5: IP (transfected 293 + anti-V5 mAb + Protein G agarose)

IF analysis of 293 cells transfected with a V5-tag protein,1:2000 dilution (blue DAPI, red anti-V5)

產(chǎn)品詳情

產(chǎn)品描述：

V5-Tag monoclonal antibody CSB-MA000160 was produced in mouse immunized by using Synthetic Peptide as the immunogen. The V5 tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD).
This V5-Tag Monoclonal Antibody was tested in the ELISA, WB, IF and IHC applications. V5-Tag is one of the epitope tags, which provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available.
宿主：

Mouse
反應(yīng)種屬：

N/A
免疫原：

Synthetic Peptide
標(biāo)記方式：

Non-conjugated
抗體亞型：

IgG
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
產(chǎn)品提供形式：

Liquid
應(yīng)用范圍：

ELISA, WB, IF, IP

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:5000
IF	1:50-1:200
IP	1:200-1:2000

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunofluorescence (IF) Protocol
Immunoprecipitation (IP) Protocol
儲(chǔ)存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.